Author: Vieira, Flávia V.; Hoffmann, Daniel J.; Fabri, Carolina U.F.; Bresciani, Katia D.S.; Gameiro, Roberto; Flores, Eduardo F.; Cardoso, Tereza C.
Title: Circulation of canine parvovirus among dogs living in human-wildlife interface in the Atlantic forest biome, Brazil Document date: 2018_1_11
ID: sybnnma7_6
Snippet: For detection of the CPV genome, total DNA from infected/uninfected AF-72 cells was extracted using DNAzol TM according to the manufacturer's instructions (Invitrogen ® ). An average of 100 ng of genomic DNA was used for PCR as described previously. Polymerase chain reaction (PCR) was performed to amplify a fragment of 583 base pairs (bp) of the VP2 gene, a region surrounding position 426, using primers described previously (Decaro et al., 2009;.....
Document: For detection of the CPV genome, total DNA from infected/uninfected AF-72 cells was extracted using DNAzol TM according to the manufacturer's instructions (Invitrogen ® ). An average of 100 ng of genomic DNA was used for PCR as described previously. Polymerase chain reaction (PCR) was performed to amplify a fragment of 583 base pairs (bp) of the VP2 gene, a region surrounding position 426, using primers described previously (Decaro et al., 2009; Mohan Raj et al., 2010) . CPV amplicons were purified using the NucleoSpin Extract II kit and sequenced with an ABI PRISM 3100 Genetic Analyser (Applied Biosystems TM ) using the BigDye Terminator v. 3.1Cycle Sequencing Kit (Applied Biosystems TM ).
Search related documents:
Co phrase search for related documents- base pair and chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- base pair and genomic dna: 1
- base pair and PCR polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- base pair and polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- bp base pair and chain reaction: 1, 2, 3
- bp base pair and PCR polymerase chain reaction: 1, 2
- bp base pair and polymerase chain reaction: 1, 2, 3
- chain reaction and genomic dna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and PCR polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- chain reaction and polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- Extract II kit and NucleoSpin Extract II kit: 1, 2, 3
- genomic dna and infected total dna: 1
- genomic dna and PCR polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
- genomic dna and polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date