Selected article for: "chain reaction and PCR base"

Author: Vieira, Flávia V.; Hoffmann, Daniel J.; Fabri, Carolina U.F.; Bresciani, Katia D.S.; Gameiro, Roberto; Flores, Eduardo F.; Cardoso, Tereza C.
Title: Circulation of canine parvovirus among dogs living in human-wildlife interface in the Atlantic forest biome, Brazil
  • Document date: 2018_1_11
  • ID: sybnnma7_6
    Snippet: For detection of the CPV genome, total DNA from infected/uninfected AF-72 cells was extracted using DNAzol TM according to the manufacturer's instructions (Invitrogen ® ). An average of 100 ng of genomic DNA was used for PCR as described previously. Polymerase chain reaction (PCR) was performed to amplify a fragment of 583 base pairs (bp) of the VP2 gene, a region surrounding position 426, using primers described previously (Decaro et al., 2009;.....
    Document: For detection of the CPV genome, total DNA from infected/uninfected AF-72 cells was extracted using DNAzol TM according to the manufacturer's instructions (Invitrogen ® ). An average of 100 ng of genomic DNA was used for PCR as described previously. Polymerase chain reaction (PCR) was performed to amplify a fragment of 583 base pairs (bp) of the VP2 gene, a region surrounding position 426, using primers described previously (Decaro et al., 2009; Mohan Raj et al., 2010) . CPV amplicons were purified using the NucleoSpin Extract II kit and sequenced with an ABI PRISM 3100 Genetic Analyser (Applied Biosystems TM ) using the BigDye Terminator v. 3.1Cycle Sequencing Kit (Applied Biosystems TM ).

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