Author: Xing, Liang; Sun, Feng; Wang, Zhendong; Li, Yan; Yang, Zhifang; Wang, Fengshan; Zhai, Guangxi; Tan, Haining
Title: Characterization and bioactivity of self-assembled anti-angiogenic chondroitin sulfate-ES2-AF nanoparticle conjugate Document date: 2019_4_10
ID: x8f598x2_34
Snippet: The pH value of the most suitable coupling reaction was screened by pre-enrichment experiments to ensure that sufficient CD44 protein was coupled onto the CM5 chip to facilitate the next screening of drugs. The experimental results showed that when the pH value of the CD44 protein solution was 3.8, the response value of the protein-chip binding was the highest; pH 3.8 was thus taken as the ideal ligand coupling condition ( Figure 5A ). Ligand con.....
Document: The pH value of the most suitable coupling reaction was screened by pre-enrichment experiments to ensure that sufficient CD44 protein was coupled onto the CM5 chip to facilitate the next screening of drugs. The experimental results showed that when the pH value of the CD44 protein solution was 3.8, the response value of the protein-chip binding was the highest; pH 3.8 was thus taken as the ideal ligand coupling condition ( Figure 5A ). Ligand conjugation was performed under these conditions with a 10 μg/mL concentration of the coupled protein CD44 solution, and the final protein coupling amount was 394.8 RU.
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