Author: Malboeuf, Christine M.; Yang, Xiao; Charlebois, Patrick; Qu, James; Berlin, Aaron M.; Casali, Monica; Pesko, Kendra N.; Boutwell, Christian L.; DeVincenzo, John P.; Ebel, Gregory D.; Allen, Todd M.; Zody, Michael C.; Henn, Matthew R.; Levin, Joshua Z.
Title: Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification Document date: 2012_9_8
ID: s76c5ebd_35
Snippet: Next, we assessed the read coverage for the serial dilution of the WNV clone relative to a WNV reference. Our method captured the entire WNV CDS with as little as 100 copies of input RNA ( Figure 1A ). In all 16 dilutions of WNV clone RNA, complete coverage was obtained (Supplementary Figure S1 ). In addition, the pattern of variable coverage was very reproducible for WNV clone with input amounts of ranging from 100 to 10 000 copies (Supplementar.....
Document: Next, we assessed the read coverage for the serial dilution of the WNV clone relative to a WNV reference. Our method captured the entire WNV CDS with as little as 100 copies of input RNA ( Figure 1A ). In all 16 dilutions of WNV clone RNA, complete coverage was obtained (Supplementary Figure S1 ). In addition, the pattern of variable coverage was very reproducible for WNV clone with input amounts of ranging from 100 to 10 000 copies (Supplementary Figure S2) . Finally, we assessed the read coverage for two RSV clinical samples. Our method captured the entire RSV CDS for both samples (Supplementary Figure S1C) . Similarly to HIV and WNV samples, the coverage is variable across the CDS.
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