Author: Jabado, Omar J.; Liu, Yang; Conlan, Sean; Quan, P. Lan; Hegyi, Hédi; Lussier, Yves; Briese, Thomas; Palacios, Gustavo; Lipkin, W. I.
Title: Comprehensive viral oligonucleotide probe design using conserved protein regions Document date: 2007_12_13
ID: xfzhn1n1_20
Snippet: Because it is not feasible to test all probes with all known viruses, we tested probe validity using a Gibbs free energy model of hybridization. All probe sequences were compared to the non-redundant set of viral sequences by BLASTN (37) . Probe-target pairs were aligned by Smith-Waterman to ensure accuracy; mismatches and change in Gibbs free energy at 658C (hybridization temperature) were then calculated......
Document: Because it is not feasible to test all probes with all known viruses, we tested probe validity using a Gibbs free energy model of hybridization. All probe sequences were compared to the non-redundant set of viral sequences by BLASTN (37) . Probe-target pairs were aligned by Smith-Waterman to ensure accuracy; mismatches and change in Gibbs free energy at 658C (hybridization temperature) were then calculated.
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