Comparison of the Luminex xTAG Respiratory Viral Panel Fast v2 Assay With Anyplex II RV16 Detection Kit and AdvanSure RV Real-Time RT-PCR Assay for the Detection of Respiratory Viruses

Ko, Dae-Hyun; Kim, Hyun Soo; Hyun, Jungwon; Kim, Han-Sung; Kim, Jae-Seok; Park, Kyoung Un; Song, Wonkeun

Luminex xTAG RVP Fast v2 assays were performed on the collected specimens or stored nucleic acids after thawing. If the Luminex xTAG RVP results were inconsistent with the results of the Anyplex II RV16 assay, then we repeated the Anyplex II RV16 assay for the same specimens. If the repeated Anyplex II RV16 results were different from the results of the first assay, then the Luminex xTAG RVP Fast v2 tests were repeated and/or a third PCR assay, i.e., the AdvanSure RV real-time RT-PCR assay was performed for comparison. The Anyplex II RV16 and AdvanSure RV real-time RT-PCR assays were performed by using the CFX96 real-time PCR system (Bio-Rad Laboratories, Hercules, CA, USA) and SLAN-96 real-time PCR detection system (LG Life Sciences), respectively. For specimens with complete results from all three assays, we considered that the results were more likely to be true when two of the three assays showed consistent results. All of the tests were performed according to the manufacturers' recommendations. The characteristics and detectable viruses of each kit are summarized in Table 1 .