Author: Chen, Wei-Ju; Arnold, John; Fairchok, Mary; Hansen, Erin; Malone, Leslie; Grigorenko, Elena; Stalons, Donald; Milzman, Jacqueline Owens; Ridore, Michelande; Coles, Christian; Burgess, Timothy; Millar, Eugene
Title: Comparative Evaluation of Diatherix Target Enriched Multiplex Polymerase Chain Reaction and BioFire FilmArray in the Detection of Viral and Bacterial Respiratory Pathogens Document date: 2016_10_24
ID: sl3w5my1_2
Snippet: Methods. Specimens (n = 403) were derived from an observational study of febrile ILI among otherwise healthy subjects at 5 US military hospitals from 2009 to 2014. Specimens were first tested by single-plex polymerase chain reaction (PCR) for influenza. Specimens were then tested by 2 assays: (1) targetâ€enriched multiplex PCR (TEM-PCR; Diatherix Laboratories, Inc, Huntsville, AL) and (2) BioFire FilmArray Respiratory Panel (FilmArray; BioFire D.....
Document: Methods. Specimens (n = 403) were derived from an observational study of febrile ILI among otherwise healthy subjects at 5 US military hospitals from 2009 to 2014. Specimens were first tested by single-plex polymerase chain reaction (PCR) for influenza. Specimens were then tested by 2 assays: (1) targetâ€enriched multiplex PCR (TEM-PCR; Diatherix Laboratories, Inc, Huntsville, AL) and (2) BioFire FilmArray Respiratory Panel (FilmArray; BioFire Diagnostics, Salt Lake City, UT). The panels have 8 viral (human rhinovirus/enterovirus [HRV/EV], influenza A, influenza B, coronavirus, respiratory syncytial virus (RSV), parainfluenza, human metapneumovirus [hMPV], adenovirus [ADV]) and 3 bacterial (Mycoplasma pneumoniae, Bordetella pertussis, and Chlamydophila pneumoniae) pathogens in common. Specimens were selected based on availability, and tests were performed in separate laboratories. Kappa (κ) coefficients and 95% confidence intervals (CIs) were computed.
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