Author: Ko, Dae-Hyun; Kim, Hyun Soo; Hyun, Jungwon; Kim, Han-Sung; Kim, Jae-Seok; Park, Kyoung Un; Song, Wonkeun
Title: Comparison of the Luminex xTAG Respiratory Viral Panel Fast v2 Assay With Anyplex II RV16 Detection Kit and AdvanSure RV Real-Time RT-PCR Assay for the Detection of Respiratory Viruses Document date: 2017_6_20
ID: vic6rbnb_20
Snippet: In the present study, we evaluated the ability of the Luminex xTAG RVP Fast v2 assay to detect common respiratory pathogens. Its performance was compared with that of the Anyplex II RV16 assay, and any discrepancies in the results were confirmed by using a third multiplex PCR assay, AdvanSure RV. In general, the Anyplex II RV16 assay detected more pathogens than did the Luminex xTAG RVP Fast v2 assay, which was not surprising considering that onl.....
Document: In the present study, we evaluated the ability of the Luminex xTAG RVP Fast v2 assay to detect common respiratory pathogens. Its performance was compared with that of the Anyplex II RV16 assay, and any discrepancies in the results were confirmed by using a third multiplex PCR assay, AdvanSure RV. In general, the Anyplex II RV16 assay detected more pathogens than did the Luminex xTAG RVP Fast v2 assay, which was not surprising considering that only specimens that showed at least one posi-tive result by the Anyplex II RV16 assay were collected for this comparison. Nonetheless, the Luminex xTAG RVP v2 assay detected more metapneumovirus and parainfluenza type 3 specimens. The xTAG RVP v2 and Anyplex II RV16 assays showed relatively poor concordance for adenovirus, coronavirus NL63, and parainfluenza type 3 specimens. Taking into account the results of the third assay, we found that the Anyplex II RV16 assay detected more adenovirus, bocavirus, and respiratory syncytial virus type A specimens. Moreover, metapneumovirus, parainfluenza type 3, enterovirus, and rhinovirus were more likely to be detected by the Luminex xTAG RVP Fast v2 assay.
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