Author: Malboeuf, Christine M.; Yang, Xiao; Charlebois, Patrick; Qu, James; Berlin, Aaron M.; Casali, Monica; Pesko, Kendra N.; Boutwell, Christian L.; DeVincenzo, John P.; Ebel, Gregory D.; Allen, Todd M.; Zody, Michael C.; Henn, Matthew R.; Levin, Joshua Z.
Title: Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification Document date: 2012_9_8
ID: s76c5ebd_2
Snippet: Development of high-throughput methods for viral sequencing from low-copy viral samples presents challenges. First, the method should be robust in generating sufficient material for sequencing libraries. Second, it should be broadly applicable to a variety of sample types. Finally, it should consistently generate sequence coverage for the entire target region, typically the protein coding region, CDS, of a viral genome......
Document: Development of high-throughput methods for viral sequencing from low-copy viral samples presents challenges. First, the method should be robust in generating sufficient material for sequencing libraries. Second, it should be broadly applicable to a variety of sample types. Finally, it should consistently generate sequence coverage for the entire target region, typically the protein coding region, CDS, of a viral genome.
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