Author: Malboeuf, Christine M.; Yang, Xiao; Charlebois, Patrick; Qu, James; Berlin, Aaron M.; Casali, Monica; Pesko, Kendra N.; Boutwell, Christian L.; DeVincenzo, John P.; Ebel, Gregory D.; Allen, Todd M.; Zody, Michael C.; Henn, Matthew R.; Levin, Joshua Z.
Title: Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification Document date: 2012_9_8
ID: s76c5ebd_38
Snippet: For selected samples from HIV, WNV and RSV, we compared the assembly statistics between three different assemblers: VICUNA, AV454 (10) and SOAPdenovo (40) (Supplementary Table S4 ). VICUNA outperformed the other assemblers by returning fewer and longer contigs that covered a larger part of the CDS. The percent of CDS covered by all contigs could not be calculated for SOAPdenovo due to the large number of short contigs which would require a second.....
Document: For selected samples from HIV, WNV and RSV, we compared the assembly statistics between three different assemblers: VICUNA, AV454 (10) and SOAPdenovo (40) (Supplementary Table S4 ). VICUNA outperformed the other assemblers by returning fewer and longer contigs that covered a larger part of the CDS. The percent of CDS covered by all contigs could not be calculated for SOAPdenovo due to the large number of short contigs which would require a second assembly in order to reconstitute a consensus sequence.
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