Author: Malboeuf, Christine M.; Yang, Xiao; Charlebois, Patrick; Qu, James; Berlin, Aaron M.; Casali, Monica; Pesko, Kendra N.; Boutwell, Christian L.; DeVincenzo, John P.; Ebel, Gregory D.; Allen, Todd M.; Zody, Michael C.; Henn, Matthew R.; Levin, Joshua Z.
Title: Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification Document date: 2012_9_8
ID: s76c5ebd_44
Snippet: In our study, we were not able to use the Ovation RNA-Seq method to quantify the amount of viral RNA amounts in our clinical samples. For RSV and HIV clinical sample, the clinical samples with highest input viral RNA amounts had the highest percent of reads aligning to viral reference (Supplementary Table S1 ), but there was no correlation between input RNA and viral sequence coverage. This method may provide approximation of viral amounts in cli.....
Document: In our study, we were not able to use the Ovation RNA-Seq method to quantify the amount of viral RNA amounts in our clinical samples. For RSV and HIV clinical sample, the clinical samples with highest input viral RNA amounts had the highest percent of reads aligning to viral reference (Supplementary Table S1 ), but there was no correlation between input RNA and viral sequence coverage. This method may provide approximation of viral amounts in clinical sample, but it is not quantitative.
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