Author: Malboeuf, Christine M.; Yang, Xiao; Charlebois, Patrick; Qu, James; Berlin, Aaron M.; Casali, Monica; Pesko, Kendra N.; Boutwell, Christian L.; DeVincenzo, John P.; Ebel, Gregory D.; Allen, Todd M.; Zody, Michael C.; Henn, Matthew R.; Levin, Joshua Z.
Title: Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification Document date: 2012_9_8
ID: s76c5ebd_48
Snippet: outbreaks (48) . Additionally, one would be able to identify and study multiple viruses from a single sample. This could be beneficial in understanding the dynamics of viral co-infection, such as HIV/HCV. Furthermore, these methods could be applied to parasite, bacterial and fungal pathogen discovery by sequencing total RNA that contains expressed pathogen RNA. Expressed pathogens could be discovered incidentally through RNA-Seq data. Additionall.....
Document: outbreaks (48) . Additionally, one would be able to identify and study multiple viruses from a single sample. This could be beneficial in understanding the dynamics of viral co-infection, such as HIV/HCV. Furthermore, these methods could be applied to parasite, bacterial and fungal pathogen discovery by sequencing total RNA that contains expressed pathogen RNA. Expressed pathogens could be discovered incidentally through RNA-Seq data. Additionally, it may be more cost effective for some projects to sequence RNA rather than DNA to detect pathogens because greater sequencing depth may be needed for DNA than RNA (24). Morin et al. have demonstrated the capability of detecting expressed pathogen RNA through RNA sequencing (49) .
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