Author: Gadsby, Naomi J.; Russell, Clark D.; McHugh, Martin P.; Mark, Harriet; Conway Morris, Andrew; Laurenson, Ian F.; Hill, Adam T.; Templeton, Kate E.
Title: Comprehensive Molecular Testing for Respiratory Pathogens in Community-Acquired Pneumonia Document date: 2016_4_1
ID: s04e6po9_3
Snippet: Quantitative molecular assays for typical respiratory bacteria have been used previously in patients with pneumonia, but these assays lack a number of key targets, use targets that have been shown to lack specificity, or may be only semiquantitative [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] . However, our group recently developed a suitable assay that is capable of quantifying bacterial loads of 8 typica.....
Document: Quantitative molecular assays for typical respiratory bacteria have been used previously in patients with pneumonia, but these assays lack a number of key targets, use targets that have been shown to lack specificity, or may be only semiquantitative [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] . However, our group recently developed a suitable assay that is capable of quantifying bacterial loads of 8 typical bacterial pathogens from lower respiratory tract (LRT) specimens [33] . This assay was extensively validated on clinical isolates, reference strains, and clinical specimens such as sputa and bronchoalveolar lavage fluids, and all bacterial targets could be reliably quantified, even when present in mixtures of different concentrations [33] .
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