Author: Zhang, XiZhen; Wang, XiaoDan; Zhao, DongHai; Meng, XiangYu; Zhao, XingHong; Yu, XiangHui; Kong, Wei
Title: Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine Document date: 2011_12_16
ID: xxypq3wd_27
Snippet: To investigate the synthesis and assembly of HIV-1 gagpol and env protein, we collected supernatant from positive cells by centrifuging at 26000 r min ï€1 at 4ï‚°C, for 4 h through a 10%-40% sucrose gradient. We found that the gagpol and env proteins passed through the lower density fractions and migrated to the 40% and 30% density layers. Western blot analysis indicated that protein could assemble into VLPs that included gagpol and env ( Figure.....
Document: To investigate the synthesis and assembly of HIV-1 gagpol and env protein, we collected supernatant from positive cells by centrifuging at 26000 r min ï€1 at 4ï‚°C, for 4 h through a 10%-40% sucrose gradient. We found that the gagpol and env proteins passed through the lower density fractions and migrated to the 40% and 30% density layers. Western blot analysis indicated that protein could assemble into VLPs that included gagpol and env ( Figure 3 ).
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