Selected article for: "standard rna and viral rna"
Author: Malboeuf, Christine M.; Yang, Xiao; Charlebois, Patrick; Qu, James; Berlin, Aaron M.; Casali, Monica; Pesko, Kendra N.; Boutwell, Christian L.; DeVincenzo, John P.; Ebel, Gregory D.; Allen, Todd M.; Zody, Michael C.; Henn, Matthew R.; Levin, Joshua Z.
Title: Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification
  • Document date: 2012_9_8
    • ID: s76c5ebd_27
    Snippet: We generated Illumina libraries for HIV, RSV and WNV samples using the Ovation RNA-Seq system with viral samples containing 500 ag to 240 fg ($100 to $30 000 copies) of viral RNA. The concentration of extracted RNA could not be quantified using standard RNA quantification methods; therefore the quantity of viral genomes from each extraction was determined by viral-specific qRT-PCR assays (Supplementary Table S1 ). For all samples, the amount of h.....
    Document: We generated Illumina libraries for HIV, RSV and WNV samples using the Ovation RNA-Seq system with viral samples containing 500 ag to 240 fg ($100 to $30 000 copies) of viral RNA. The concentration of extracted RNA could not be quantified using standard RNA quantification methods; therefore the quantity of viral genomes from each extraction was determined by viral-specific qRT-PCR assays (Supplementary Table S1 ). For all samples, the amount of host RNA was determined by 18S rRNA qRT-PCR assays (Supplementary Table S1 ). We used Illumina sequencing to ensure that we obtained sufficient coverage to compensate for the high levels of host contamination (discussed later) in the clinical samples.

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