Selected article for: "Golgi complex and plasma membrane"
Author: Benharouga, Mohamed; Haardt, Martin; Kartner, Norbert; Lukacs, Gergely L.
Title: Cooh-Terminal Truncations Promote Proteasome-Dependent Degradation of Mature Cystic Fibrosis Transmembrane Conductance Regulator from Post-Golgi Compartments
  • Document date: 2001_5_28
    • ID: q3agdeju_21
    Snippet: BHK cells expressing wt or T70 CFTR were incubated in the presence of cycloheximide (100 g/ml) to ensure the degradation of the core-glycosylated wt and T70 CFTR (Lukacs et al., 1994) . Isolation of ER-, Golgi complex-, and plasma membrane-enriched microsomes was performed as described (Zhang et al., 1998) . The microsomes (0.8-1.5 mg/ml) were digested in HSE medium (10 mM Hepes, 0.25 M sucrose, pH 7.6) in the presence, at the indicated concentra.....
    Document: BHK cells expressing wt or T70 CFTR were incubated in the presence of cycloheximide (100 g/ml) to ensure the degradation of the core-glycosylated wt and T70 CFTR (Lukacs et al., 1994) . Isolation of ER-, Golgi complex-, and plasma membrane-enriched microsomes was performed as described (Zhang et al., 1998) . The microsomes (0.8-1.5 mg/ml) were digested in HSE medium (10 mM Hepes, 0.25 M sucrose, pH 7.6) in the presence, at the indicated concentration, of trypsin or proteinase K for 15 min at 4 Њ C (Zhang et al., 1998) . Proteolysis was terminated by the addition of 1 mM PMSF. Samples were immediately denatured in 2 ϫ Laemmli sample buffer at 37 Њ C for 20 min and probed by immunoblot analysis.

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