Author: Feng, Mingqian; Bian, Hejiao; Wu, Xiaolin; Fu, Tianyun; Fu, Ying; Hong, Jessica; Fleming, Bryan D; Flajnik, Martin F; Ho, Mitchell
Title: Construction and next-generation sequencing analysis of a large phage-displayed V(NAR) single-domain antibody library from six naïve nurse sharks Document date: 2018_11_7
ID: wc6k06sm_14
Snippet: In the present study, we developed the EASeL method to construct phage display libraries based on PCR extension assembly followed by self-ligation. By using this EASeL method, we successfully constructed a large phagedisplayed V NAR library with a size of 10 10 from six antigen-Antibody Therapeutics, 2019 5 We also demonstrated that Type I and Type II shark V NAR binders can be produced from E. coli as functional single-domain proteins. The large.....
Document: In the present study, we developed the EASeL method to construct phage display libraries based on PCR extension assembly followed by self-ligation. By using this EASeL method, we successfully constructed a large phagedisplayed V NAR library with a size of 10 10 from six antigen-Antibody Therapeutics, 2019 5 We also demonstrated that Type I and Type II shark V NAR binders can be produced from E. coli as functional single-domain proteins. The large nurse shark V NAR singledomain phage library described here provides an attractive single-domain antibody platform for drug discovery.
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