Author: Chen, Haifeng; Mammel, Mark; Kulka, Mike; Patel, Isha; Jackson, Scott; Goswami, Biswendu B.
Title: Detection and Identification of Common Food-Borne Viruses with a Tiling Microarray Document date: 2011_5_16
ID: ycs5rtoc_20
Snippet: Our hybridization data clearly show that unamplified target cDNA synthesized by reverse transcription and labeled postsynthetically (Supplement) can be hybridized to probes in a high density array and individual virus strains can be identified either by comparison to a reference strain or by the hybridization profile of the entire array ( Fig. 1A-D) regardless of their genome sequence. The high sensitivity of the assay is achieved by two cycles o.....
Document: Our hybridization data clearly show that unamplified target cDNA synthesized by reverse transcription and labeled postsynthetically (Supplement) can be hybridized to probes in a high density array and individual virus strains can be identified either by comparison to a reference strain or by the hybridization profile of the entire array ( Fig. 1A-D) regardless of their genome sequence. The high sensitivity of the assay is achieved by two cycles of hybridization signal amplification as described in Methods. The detection limit is approximately 10,000 viral genomes.
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