Author: MINAMI-FUKUDA, Fujiko; NAGAI, Makoto; TAKAI, Hikaru; MURAKAMI, Toshiaki; OZAWA, Tadashi; TSUCHIAKA, Shinobu; OKAZAKI, Sachiko; KATAYAMA, Yukie; OBA, Mami; NISHIURA, Naomi; SASSA, Yukiko; OMATSU, Tsutomu; FURUYA, Tetsuya; KOYAMA, Satoshi; SHIRAI, Junsuke; TSUNEMITSU, Hiroshi; FUJII, Yoshiki; KATAYAMA, Kazuhiko; MIZUTANI, Tetsuya
Title: Detection of Bovine Group A Rotavirus Using Rapid Antigen Detection Kits, RT-PCR and Next-Generation DNA Sequencing Document date: 2013_8_2
ID: vsjy9oaf_3
Snippet: Three bovine RVA strains (IS1 (G6P [5] ), NCDV (G6P [1] ) and KK3 (G10P [11] )) were subjected to study of sensitivity of detection. An established fetal rhesus monkey cell line (MA-104) was used for virus propagation, and culture fluids were used for tests. The titers of each virus stocks were 10 7.0 TCID 50 /ml. Thirteen fecal samples were taken twice daily from seven beef farms located in Ishikawa Prefecture, Japan, that had an epidemic histor.....
Document: Three bovine RVA strains (IS1 (G6P [5] ), NCDV (G6P [1] ) and KK3 (G10P [11] )) were subjected to study of sensitivity of detection. An established fetal rhesus monkey cell line (MA-104) was used for virus propagation, and culture fluids were used for tests. The titers of each virus stocks were 10 7.0 TCID 50 /ml. Thirteen fecal samples were taken twice daily from seven beef farms located in Ishikawa Prefecture, Japan, that had an epidemic history of RVA. These specimens were collected between 2012 and 2013 from calves (aged<1 year) that showed watery or sticky diarrhea that was white, yellow or greenish brown.
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