Author: Paquin, Ashley; Onabajo, Olusegun O.; Tang, Wei; Prokunina-Olsson, Ludmila
Title: Comparative Functional Analysis of 12 Mammalian IFN-?4 Orthologs Document date: 2016_1_1
ID: sqxrwgif_21
Snippet: For initial screening, transfections were done for 48 h in 6-well plates with 350,000 cells/well. Constructs, which showed positive signals for MAB-IFN-l4, were further evaluated by transfections in 12-well plates with 200,000 cells/well, in triplicates. Cells were harvested, fixed for 30 min at room temperature with 100 mL of cytofix (BD Biosciences), and stained overnight in cytoperm (BD Biosciences) with 0.5 mg/mL of a-Halo, 1 mg/mL RAB-IFN-l4.....
Document: For initial screening, transfections were done for 48 h in 6-well plates with 350,000 cells/well. Constructs, which showed positive signals for MAB-IFN-l4, were further evaluated by transfections in 12-well plates with 200,000 cells/well, in triplicates. Cells were harvested, fixed for 30 min at room temperature with 100 mL of cytofix (BD Biosciences), and stained overnight in cytoperm (BD Biosciences) with 0.5 mg/mL of a-Halo, 1 mg/mL RAB-IFN-l4, or 2 mg/mL of MAB-IFN-l4. Secondary antibodies were donkey anti-mouse and donkey anti-rabbit Alexa Fluor 488 (1 mg/mL; Life Technologies). Cells were analyzed using multiparametric flow cytometry on a FACS Aria III (BD Biosciences) and FlowJo.10 software (Tree Star).
Search related documents:
Co phrase search for related documents- anti rabbit and flow cytometry: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- anti rabbit and overnight stain: 1
- anti rabbit and positive signal: 1, 2, 3, 4
- anti rabbit and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57
- anti rabbit and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58
- BD Biosciences cytoperm and flow cytometry: 1, 2
- BD Biosciences cytoperm and room temperature: 1, 2
- flow cytometry and multiparametric flow cytometry: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- flow cytometry and plate transfection: 1
- flow cytometry and positive signal: 1
- flow cytometry and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- flow cytometry and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- flow cytometry and Tree Star software: 1, 2, 3, 4
- overnight stain and room temperature: 1
- overnight stain and secondary antibody: 1
- positive signal and room temperature: 1, 2
- positive signal and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- room temperature and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42
- room temperature and Tree Star software: 1, 2
Co phrase search for related documents, hyperlinks ordered by date