Selected article for: "PCR method and RT PCR method"
Author: TSAI, Cheng-Ta; TSAI, Hsin-Fu; WANG, Ching-Ho
Title: Detection of infectious bronchitis virus strains similar to Japan in Taiwan
  • Document date: 2016_1_29
    • ID: xvos1qto_5
    Snippet: The samples that were IBV positive with the N-specific primer were subjected to RT-PCR and sequencing using the S-specific primer, oligo5/rC3-r for genotyping [17] . The sequences of these primers are as follows: oligo5: 5′-AAACT GAACA AAAGA CAGAC TTAG-3′ (20251-20274, H120 numbering, GenBank accession number: FJ888351) and rC3-r: 5′-(A/G) CAAT GTGTA ACAAA (T/C) ACT-3′ (20655-20637). The same RT-PCR procedure was performed, except the ann.....
    Document: The samples that were IBV positive with the N-specific primer were subjected to RT-PCR and sequencing using the S-specific primer, oligo5/rC3-r for genotyping [17] . The sequences of these primers are as follows: oligo5: 5′-AAACT GAACA AAAGA CAGAC TTAG-3′ (20251-20274, H120 numbering, GenBank accession number: FJ888351) and rC3-r: 5′-(A/G) CAAT GTGTA ACAAA (T/C) ACT-3′ (20655-20637). The same RT-PCR procedure was performed, except the annealing temperature for this primer set was 52°C. For LC field cases, one trachea from each flock was subjected to RT-PCR using the same method described for broilers. The prevalence differences among different seasons or chicken types were analyzed using chi-square tests or Fisher's exact tests when any cell having expected value less than 5 [12] . The level of a significant difference was set at α 0.05.

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