Author: Azhar, Esam I.; Hashem, Anwar M.; El-Kafrawy, Sherif A.; Sohrab, Sayed Sartaj; Aburizaiza, Asad S.; Farraj, Suha A.; Hassan, Ahmed M.; Al-Saeed, Muneera S.; Jamjoom, Ghazi A.; Madani, Tariq A.
Title: Detection of the Middle East Respiratory Syndrome Coronavirus Genome in an Air Sample Originating from a Camel Barn Owned by an Infected Patient Document date: 2014_7_22
ID: ynlj4cge_8
Snippet: Real-time RT-PCR. RNA was extracted from the dissolved filter solution using a QIAamp viral RNA minikit (Qiagen, Germany) according to manufacturer's instructions. Eluted RNA was screened for the UpE region using the real-time RT-PCR assay on a Rotor-Gene Q real-time PCR machine (Qiagen, Germany) as previously described (22) . Samples were also tested by real-time RT-PCR for the ORF1a and ORF1b regions for confirmation as described previously (22.....
Document: Real-time RT-PCR. RNA was extracted from the dissolved filter solution using a QIAamp viral RNA minikit (Qiagen, Germany) according to manufacturer's instructions. Eluted RNA was screened for the UpE region using the real-time RT-PCR assay on a Rotor-Gene Q real-time PCR machine (Qiagen, Germany) as previously described (22) . Samples were also tested by real-time RT-PCR for the ORF1a and ORF1b regions for confirmation as described previously (22) .
Search related documents:
Co phrase search for related documents- Germany Qiagen viral RNA minikit and RNA minikit: 1
- Germany Qiagen viral RNA minikit and viral RNA minikit: 1
- manufacturer instruction and real time: 1
- ORF1b region and real time: 1, 2, 3, 4, 5, 6
- ORF1b region and RT PCR assay: 1, 2, 3
- ORF1b region and UpE region: 1
- pcr machine and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- pcr machine and real time pcr machine: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- pcr machine and real time RT PCR test: 1
- pcr machine and RT PCR assay: 1, 2, 3
- previously describe and real time: 1, 2
- previously describe and real time RT PCR test: 1
- QIAamp Germany Qiagen viral RNA minikit and RNA minikit: 1
- QIAamp Germany Qiagen viral RNA minikit and viral RNA minikit: 1
- real time and RNA minikit: 1, 2, 3
- real time and RT PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- real time and sample real time RT PCR test: 1, 2, 3, 4, 5, 6
- real time and UpE region: 1, 2, 3, 4, 5, 6, 7, 8
- real time and viral RNA minikit: 1
Co phrase search for related documents, hyperlinks ordered by date