Selected article for: "gene site and recombinant baculovirus"
Author: Liu, Xingjian; Yang, Xin; Mehboob, Arslan; Hu, Xiaoyuan; Yi, Yongzhu; Li, Yinü; Zhang, Zhifang
Title: A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons
  • Document date: 2019_12_19
    • ID: uxzeg16r_10
    Snippet: According to a published protocol (Datsenko & Wanner, 2000; Liu et al., 2016) , the p10 gene of reBmBac baculovirus genomic DNA was replaced by the rpsL-neo cassette by phage λ-Red recombinase in E. coli. Then, the rpsL-neo cassette was replaced by the chIFN-γ gene. The recombinant baculovirus shuttle vector containing the chIFN-γ gene at the p10 gene site was named reBmBac-p10Cγ......
    Document: According to a published protocol (Datsenko & Wanner, 2000; Liu et al., 2016) , the p10 gene of reBmBac baculovirus genomic DNA was replaced by the rpsL-neo cassette by phage λ-Red recombinase in E. coli. Then, the rpsL-neo cassette was replaced by the chIFN-γ gene. The recombinant baculovirus shuttle vector containing the chIFN-γ gene at the p10 gene site was named reBmBac-p10Cγ.

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