Author: Usui, Kimihito; Ichihashi, Norikazu; Yomo, Tetsuya
Title: A design principle for a single-stranded RNA genome that replicates with less double-strand formation Document date: 2015_9_18
ID: znhvdtg8_33
Snippet: 45 110 a RNA replication was performed with each mdv-⣠mutant (100 nM) and Q⤠replicase (100 nM) for 20 min at 37 • C, and the total RNA synthesis and the dsRNA ratios were measured as described in 'Materials and Methods' section. The mutants that exhibit <0.5 dsRNA ratios are shadowed. b The loops were reduced by synonymous substitution rather than deletion......
Document: 45 110 a RNA replication was performed with each mdv-⣠mutant (100 nM) and Q⤠replicase (100 nM) for 20 min at 37 • C, and the total RNA synthesis and the dsRNA ratios were measured as described in 'Materials and Methods' section. The mutants that exhibit <0.5 dsRNA ratios are shadowed. b The loops were reduced by synonymous substitution rather than deletion.
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