Selected article for: "primer extension and secondary structure"
Author: Usui, Kimihito; Ichihashi, Norikazu; Yomo, Tetsuya
Title: A design principle for a single-stranded RNA genome that replicates with less double-strand formation
  • Document date: 2015_9_18
    • ID: znhvdtg8_40
    Snippet: There is a limitation in the design rule derived in this study. The design method according to the 'less GC in loops' rule presently depends on secondary structure prediction, which is reported as valid for only small RNAs of typically <500 nt (40) . For the design of longer ssRNAs, the development of more reliable prediction algorithms or a combination of experimental methods to detect loop structures, such as Selective 2 -hydroxyl acylation ana.....
    Document: There is a limitation in the design rule derived in this study. The design method according to the 'less GC in loops' rule presently depends on secondary structure prediction, which is reported as valid for only small RNAs of typically <500 nt (40) . For the design of longer ssRNAs, the development of more reliable prediction algorithms or a combination of experimental methods to detect loop structures, such as Selective 2 -hydroxyl acylation analyzed by primer extension (SHAPE), (41) would be required.

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