Author: Al-Mulla, Hawaa M. N.; Turrell, Lauren; Smith, Nicola M.; Payne, Luke; Baliji, Surendranath; Züst, Roland; Thiel, Volker; Baker, Susan C.; Siddell, Stuart G.; Neuman, Benjamin W.
Title: Competitive Fitness in Coronaviruses Is Not Correlated with Size or Number of Double-Membrane Vesicles under Reduced-Temperature Growth Conditions Document date: 2014_4_1
ID: tfuupgkg_15
Snippet: DMV capacity and RNA synthesis. Viral RNA accumulates in DMVs, leading to the interpretation that DMV clusters are the (4), although a recent study has called into question whether all DMVs are active in RNA synthesis (38) . In this case, the total DMV capacity in an infected cell may limit the amount of viral RNA synthesis that can take place inside a cell. To estimate total DMV capacity, DMV size ( Table 2) was multiplied by the relative percen.....
Document: DMV capacity and RNA synthesis. Viral RNA accumulates in DMVs, leading to the interpretation that DMV clusters are the (4), although a recent study has called into question whether all DMVs are active in RNA synthesis (38) . In this case, the total DMV capacity in an infected cell may limit the amount of viral RNA synthesis that can take place inside a cell. To estimate total DMV capacity, DMV size ( Table 2) was multiplied by the relative percentage of DMV-containing cells (Table 3) and multiplied by the number of DMVs per cluster (Table 3) to estimate the relative DMV capacity of cells infected with wild-type and mutant viruses. Results were normalized to those for wild-type controls grown at 33°C and are shown in Table 3 .
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