Author: Al-Mulla, Hawaa M. N.; Turrell, Lauren; Smith, Nicola M.; Payne, Luke; Baliji, Surendranath; Züst, Roland; Thiel, Volker; Baker, Susan C.; Siddell, Stuart G.; Neuman, Benjamin W.
Title: Competitive Fitness in Coronaviruses Is Not Correlated with Size or Number of Double-Membrane Vesicles under Reduced-Temperature Growth Conditions Document date: 2014_4_1
ID: tfuupgkg_3
Snippet: In this study, we examined the intracellular organelle phenotype of MHV strains with temperature-sensitive (ts) mutations in each of the known cistrons. Both mutant and control viruses were grown at 33°C, a temperature selected to allow full growth of mutant viruses. We chose to work with ts viruses as a pool of readymade replicase point mutants that have been preselected for both robust growth at 33°C and functional importance, since each of t.....
Document: In this study, we examined the intracellular organelle phenotype of MHV strains with temperature-sensitive (ts) mutations in each of the known cistrons. Both mutant and control viruses were grown at 33°C, a temperature selected to allow full growth of mutant viruses. We chose to work with ts viruses as a pool of readymade replicase point mutants that have been preselected for both robust growth at 33°C and functional importance, since each of the ts mutations is lethal at 40°C. We carried out these tests in order both to better understand the connection between organelles and viral RNA synthesis and to examine whether mutation of critical residues produces a detectable effect on the replicative organelle. To do this, we quantified virus release, DMV formation, RNA synthesis, and competitive fitness in a variety of cell types.
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