Title: Deletions into an NH2-terminal hydrophobic domain result in secretion of rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein Document date: 1985_12_1
ID: zrv9fjgn_14
Snippet: To examine the ultrastructural morphology of untreated COS 7-and of SA1 l-infected MA104 cells (5.5 h post-infection, infected according to published methods [12] ), coverslips were fixed in 2% glutataldehyde in 0.1 M cacodylate buffer, pH 7.4, for 45 min at 22"C. Cells were postfixed in 1% osmium tetroxide in cacodylate buffer, stained in 1% uranyl acetate, dehydrated in ethanol, and embedded in resin before thin sectioning. Sections were staine.....
Document: To examine the ultrastructural morphology of untreated COS 7-and of SA1 l-infected MA104 cells (5.5 h post-infection, infected according to published methods [12] ), coverslips were fixed in 2% glutataldehyde in 0.1 M cacodylate buffer, pH 7.4, for 45 min at 22"C. Cells were postfixed in 1% osmium tetroxide in cacodylate buffer, stained in 1% uranyl acetate, dehydrated in ethanol, and embedded in resin before thin sectioning. Sections were stained with uranyl rinse and Reynolds lead stain for several minutes (33) and then specimens were examined and photographed in a JEOL 100 CX electron microscope at 80 kV.
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