Author: Zhou, Jie; Li, Cun; Sachs, Norman; Chiu, Man Chun; Wong, Bosco Ho-Yin; Chu, Hin; Poon, Vincent Kwok-Man; Wang, Dong; Zhao, Xiaoyu; Wen, Lei; Song, Wenjun; Yuan, Shuofeng; Wong, Kenneth Kak-Yuen; Chan, Jasper Fuk-Woo; To, Kelvin Kai-Wang; Chen, Honglin; Clevers, Hans; Yuen, Kwok-Yung
Title: Differentiated human airway organoids to assess infectivity of emerging influenza virus Document date: 2018_6_26
ID: z637eh2z_26
Snippet: Flow Cytometry Analysis. To assess the percentages of four types of cells, the AOs were analyzed by flow cytometry. Briefly, the organoids were dissociated with 10 mM EDTA (Invitrogen) for 30-60 min at 37°C, fixed with 4% PFA, and permeabilized with 0.1% Triton X-100. Subsequently, the cells were incubated with primary antibodies (SI Appendix, Table S3 ) for 1 h at 4°C followed by staining with secondary antibodies. A BD FACSCanto II system was.....
Document: Flow Cytometry Analysis. To assess the percentages of four types of cells, the AOs were analyzed by flow cytometry. Briefly, the organoids were dissociated with 10 mM EDTA (Invitrogen) for 30-60 min at 37°C, fixed with 4% PFA, and permeabilized with 0.1% Triton X-100. Subsequently, the cells were incubated with primary antibodies (SI Appendix, Table S3 ) for 1 h at 4°C followed by staining with secondary antibodies. A BD FACSCanto II system was used to analyze the samples.
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