Title: Deletions into an NH2-terminal hydrophobic domain result in secretion of rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein Document date: 1985_12_1
ID: zrv9fjgn_11
Snippet: Medium from the transfected cell cultures had an equal volume of 2x lysis buffer added which contained 1 mM methionine and 1 mg/ml bovine serum albumin. Rabbit polyclonal anti-VP7 antiserum was added to each and incubated overnight at 4"C, followed by the addition of pre-absorbed PAS beads......
Document: Medium from the transfected cell cultures had an equal volume of 2x lysis buffer added which contained 1 mM methionine and 1 mg/ml bovine serum albumin. Rabbit polyclonal anti-VP7 antiserum was added to each and incubated overnight at 4"C, followed by the addition of pre-absorbed PAS beads.
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