Detection and Identification of Common Food-Borne Viruses with a Tiling Microarray

Chen, Haifeng; Mammel, Mark; Kulka, Mike; Patel, Isha; Jackson, Scott; Goswami, Biswendu B.

We have also examined whether individual virus strains can be identified by the tiling array, when multiple viruses are present in the same sample (Fig. 1E) . The ability of the microarray to accurately predict the genotypes of several different viruses led us to evaluate if the microarray can detect multiple unrelated viruses in the same experiment. We examined CXKV B3, HAV HM-175/18f and NoV #3263 RNAs as a mixture of labeled cDNA targets derived from their respective viruses hybridized to a single microarray. As shown in Fig. (1E) , the presence of CXKV B3 target and NoV#3263 can be readily identified by their strong hybridization to the set of oligoprobes derived from their individual genera sequence of CXKVB3 and L07418, respectively. Strong hybridization of HAV HM-175/18f target to the genotype IB probes is also observed in Fig. (1E) , although some cross-hybridization to its close relative genotype IA oligoprobes also occurred, due to their high sequence similarity, albeit with a much lower signal intensities.