Detection and Isolation of Exotic Newcastle Disease Virus from Field-Collected Flies

Chakrabarti, Seemanti; King, Daniel J.; Afonso, Claudio; Swayne, David; Cardona, Carol J.; Kuney, Douglas R.; Gerry, Alec C.

Flies are thus contacting infectious ENDV in the environment and are capable of harboring at least low levels of this virus. The ENDV infective dose for a chicken is reported to range from Ϸ10 3.0 (King 1996b) to 10 4.0 EID 50 (Alexander et al. 2006) , far greater than the Ͻ1 EID 50 per ßy found in our Þeld-collected ßy pools. However, given the small number of ßies collected, it would be premature to assume ßies are not capable of carrying substantially higher viral loads. It is entirely possible that these ßies had contacted a source of ENDV several days before our sampling and the low viral load represented residual virus still associated with the ßies at the time of their capture.