Detection of Bovine Group A Rotavirus Using Rapid Antigen Detection Kits, RT-PCR and Next-Generation DNA Sequencing

MINAMI-FUKUDA, Fujiko; NAGAI, Makoto; TAKAI, Hikaru; MURAKAMI, Toshiaki; OZAWA, Tadashi; TSUCHIAKA, Shinobu; OKAZAKI, Sachiko; KATAYAMA, Yukie; OBA, Mami; NISHIURA, Naomi; SASSA, Yukiko; OMATSU, Tsutomu; FURUYA, Tetsuya; KOYAMA, Satoshi; SHIRAI, Junsuke; TSUNEMITSU, Hiroshi; FUJII, Yoshiki; KATAYAMA, Kazuhiko; MIZUTANI, Tetsuya

Several rapid antigen detection (RAD) kits for human RVA are commercially available. Nemoto et al. [11] reported the usefulness of diagnosing with equine RVA of RAD kits; however, no paper has been reported for bovine RVA. The RT-PCR method of detecting VP7 with the primer pair Beg9/End9, which has been applied to each human serotype virus [4] , is usually used in livestock hygiene service centers for detection bovine RVA in Japan. Recently, Zhu et al. demonstrated a one-step duplex RT-PCR for bovine RVA amplifying the VP6 gene. In this study, we compared the sensitivity of RAD kits and RT-PCR for the detection of bovine RVA. Furthermore, we evaluated the usefulness of next-generate DNA sequencing (NGS) for direct detection from fecal samples.