Selected article for: "GAPDH internal control and PCR assay"
Author: Piewbang, Chutchai; Rungsipipat, Anudep; Poovorawan, Yong; Techangamsuwan, Somporn
Title: Cross-sectional investigation and risk factor analysis of community-acquired and hospital-associated canine viral infectious respiratory disease complex
  • Document date: 2019_11_14
    • ID: r5oefcrj_15
    Snippet: Both multiplex RT-PCR (for CIV, CPIV, CDV and CRCoV) and multiplex PCR (for CAdV-2 and CaHV-1) were performed as previously reported [26] . In addition, a first round of RT-PCR for CRCoV detection was conducted prior to performing the multiplex RT-PCR to augment the detection sensitivity. Specific oligonucleotide primers for CIV (M gene), CPIV (NP gene), CDV (NP gene), CRCoV (S gene), CAdV-1 (E3 gene), CaHV-1 (GB gene), and glyceraldehyde-3-phosp.....
    Document: Both multiplex RT-PCR (for CIV, CPIV, CDV and CRCoV) and multiplex PCR (for CAdV-2 and CaHV-1) were performed as previously reported [26] . In addition, a first round of RT-PCR for CRCoV detection was conducted prior to performing the multiplex RT-PCR to augment the detection sensitivity. Specific oligonucleotide primers for CIV (M gene), CPIV (NP gene), CDV (NP gene), CRCoV (S gene), CAdV-1 (E3 gene), CaHV-1 (GB gene), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), as an internal control, were used. The PCR primer sequences, reaction mixture, assay conditions and amplicon visualization were performed as previously reported [26] .

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