Author: Si, Yang; Zhang, Zheng; Wu, Wanrong; Fu, Qiuxia; Huang, Kang; Nitin, Nitin; Ding, Bin; Sun, Gang
Title: Daylight-driven rechargeable antibacterial and antiviral nanofibrous membranes for bioprotective applications Document date: 2018_3_16
ID: y3scrphl_29
Snippet: A daylight irradiation device (Spectrolinker XL-1500, Spectronics Corporation) equipped with a D65 standard light source (GE F15T8/D) was used in our experiment. The output power was set to an irradiance of 6.5 mW cm −2 at a distance of 16 cm from the lamp. The generation of ROS was measured in terms of the amounts of OH• and H 2 O 2 . The yield of OH• was quantified by the bleaching of p-NDA, which was a selective scavenger to quench hydro.....
Document: A daylight irradiation device (Spectrolinker XL-1500, Spectronics Corporation) equipped with a D65 standard light source (GE F15T8/D) was used in our experiment. The output power was set to an irradiance of 6.5 mW cm −2 at a distance of 16 cm from the lamp. The generation of ROS was measured in terms of the amounts of OH• and H 2 O 2 . The yield of OH• was quantified by the bleaching of p-NDA, which was a selective scavenger to quench hydroxyl radicals (36) . In a typical experiment, 10 mg of the sample membranes was immersed into 10 ml of p-NDA (50 mM), which was exposed under daylight or dark conditions for a certain time. The amount of p-NDA left in the solution was measured quantitatively with the absorbance at 440 nm with l max . Similarly, the amount of H 2 O 2 was quantified with an indirect spectrophotometric method (26) . In a typical test, 10 mg of the sample membranes was placed in 10 ml of deionized water, which was exposed under daylight or dark conditions for a certain time. After the exposure, 1 ml of sample solution was mixed with 1 ml of reagent I [aqueous solution of potassium iodide (66 g liter −1 ), sodium hydroxide (2 g liter −1 ), and ammonium molybdate tetrahydrate (0.2 g liter −1 )] and 1 ml of reagent II [aqueous solution of potassium hydrogen phthalate (20 g liter −1 )] with stirring for 5 min. The concentration of formed H 2 O 2 in the solution was measured quantitatively with the absorbance at 351 nm with l max .
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