Author: Agnihothram, Sudhakar; Yount, Boyd L.; Donaldson, Eric F.; Huynh, Jeremy; Menachery, Vineet D.; Gralinski, Lisa E.; Graham, Rachel L.; Becker, Michelle M.; Tomar, Sakshi; Scobey, Trevor D.; Osswald, Heather L.; Whitmore, Alan; Gopal, Robin; Ghosh, Arun K.; Mesecar, Andrew; Zambon, Maria; Heise, Mark; Denison, Mark R.; Baric, Ralph S.
Title: A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant Document date: 2014_3_25
ID: y4zoyqua_36
Snippet: BtCoV HKU5-SE replication and serial passage in mice. Young (10week-old) and aged (1-year-old) BALB/c mice purchased from Harlan Labs were intranasally inoculated with a 50-l volume containing 1 Ï« 10 5 PFU of BtCoV HKU5-SE virus. Infected animals were monitored for weight loss, morbidity, and clinical signs of disease, and lung titers were determined by plaque assay. Adaptation to mice was conferred by serial passage in lungs of young BALB/c mic.....
Document: BtCoV HKU5-SE replication and serial passage in mice. Young (10week-old) and aged (1-year-old) BALB/c mice purchased from Harlan Labs were intranasally inoculated with a 50-l volume containing 1 Ï« 10 5 PFU of BtCoV HKU5-SE virus. Infected animals were monitored for weight loss, morbidity, and clinical signs of disease, and lung titers were determined by plaque assay. Adaptation to mice was conferred by serial passage in lungs of young BALB/c mice (10 weeks old) at 2-day intervals p.i. as has been described in detail previously (25) . Animal housing, care, and experimental protocols were in accordance with University of North Carolina Institutional Animal Care and Use Committee (IACUC) guidelines.
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