Author: DOKI, Tomoyoshi; TAKANO, Tomomi; HOHDATSU, Tsutomu
Title: Development of a mouse-feline chimeric antibody against feline tumor necrosis factor-alpha Document date: 2016_6_4
ID: y0x0quha_10
Snippet: Purification of mouse mAb 2-4 and chimeric mAb 2-4: Mouse mAb 2-4 was purified from the hybridoma mAb 2-4 culture supernatant with Protein G Sepharose (GE Healthcare, Chicago, IL, U.S.A.) according to the product manual. Chimeric mAb 2-4 was purified from the FOCM24 culture supernatant with Protein A Sepharose (GE Healthcare). After purification, the buffer of mAbs was exchanged to PBS (pH 7.4) by Amicon Ultra-15 centrifugal filter devices (NMWL .....
Document: Purification of mouse mAb 2-4 and chimeric mAb 2-4: Mouse mAb 2-4 was purified from the hybridoma mAb 2-4 culture supernatant with Protein G Sepharose (GE Healthcare, Chicago, IL, U.S.A.) according to the product manual. Chimeric mAb 2-4 was purified from the FOCM24 culture supernatant with Protein A Sepharose (GE Healthcare). After purification, the buffer of mAbs was exchanged to PBS (pH 7.4) by Amicon Ultra-15 centrifugal filter devices (NMWL 30,000; Millipore, Billerica, MA, U.S.A.). The concentrations of purified mAbs were assayed by the Bradford method.
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