Development of a mouse-feline chimeric antibody against feline tumor necrosis factor-alpha

DOKI, Tomoyoshi; TAKANO, Tomomi; HOHDATSU, Tsutomu

Neutralizing activity of mAbs against feline TNF-alpha: Mouse mAb 2-4 and chimeric mAb 2-4 were tested for their neutralization activity against recombinant fTNF-α using the WEHI-164 cytotoxicity assay system. Mouse mAb 2-4 and chimeric mAb 2-4 neutralized the cytotoxic activity of recombinant fTNF-alpha on WEHI-164 cells in a concentration-dependent manner ( Fig. 2A) . Similarly, the neutralization activity of mouse mAb 2-4 and chimeric mAb 2-4 against endogenous fTNF-alpha contained in ascites of cats with FIP was confirmed. Mouse mAb 2-4 and chimeric mAb 2-4 neutralized the cytotoxic activity of ascites from cats with FIP on WEHI-164 cells in a concentration-dependent manner (Fig. 2B) . mAb R-G-4 (as a control for mAb 2-4) did not neutralize the cytotoxic activity of both recombinant fTNF-alpha and ascites of cats with FIP ( Fig. 2A and 2B) .