Selected article for: "PCR amplicon and phylogenetic analysis"
Author: Chen, Haifeng; Mammel, Mark; Kulka, Mike; Patel, Isha; Jackson, Scott; Goswami, Biswendu B.
Title: Detection and Identification of Common Food-Borne Viruses with a Tiling Microarray
  • Document date: 2011_5_16
    • ID: ycs5rtoc_16
    Snippet: The hybridization result from HAS 15 and PA 21, respectively, when the analysis was extended over 3.7 kb from the 5' end. Clearly HAV strains hybridized to probes from HAV strains only. Strong hybridization to the oligonucleotide probes derived from their respective subgenotype genomes represented on the array indicates the presence of these viruses. (C) Phylogenetic analysis of NoV#186 based on 300-bp sequenced PCR amplicon and other sequences f.....
    Document: The hybridization result from HAS 15 and PA 21, respectively, when the analysis was extended over 3.7 kb from the 5' end. Clearly HAV strains hybridized to probes from HAV strains only. Strong hybridization to the oligonucleotide probes derived from their respective subgenotype genomes represented on the array indicates the presence of these viruses. (C) Phylogenetic analysis of NoV#186 based on 300-bp sequenced PCR amplicon and other sequences from representative NoV strains from GenBank. Phylogenetic tree was plotted by Neighbor-joining method.

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