Author: TSAI, Cheng-Ta; TSAI, Hsin-Fu; WANG, Ching-Ho
Title: Detection of infectious bronchitis virus strains similar to Japan in Taiwan Document date: 2016_1_29
ID: xvos1qto_3
Snippet: In order to investigate the IBV prevalence and types in the field, a total of 66 broiler flocks from slaughterhouses were sampled in different seasons in 2013: 17 flocks in spring, 13 in summer, 15 in fall and 21 in winter. The broilers were at the market ages of 5 to 6 weeks old and sampled throughout Taiwan. The samples from slaughterhouses were named S before sampling numbers. Twenty tracheas were taken from each flock, and five tracheas were .....
Document: In order to investigate the IBV prevalence and types in the field, a total of 66 broiler flocks from slaughterhouses were sampled in different seasons in 2013: 17 flocks in spring, 13 in summer, 15 in fall and 21 in winter. The broilers were at the market ages of 5 to 6 weeks old and sampled throughout Taiwan. The samples from slaughterhouses were named S before sampling numbers. Twenty tracheas were taken from each flock, and five tracheas were pooled into one tube for IBV gene detection using reverse transcription polymerase chain reaction (RT-PCR). The pooled tracheas were homogenized with tryptose phosphate broth (TPB), and the nucleic acids were extracted using a commercial viral nucleic acid extraction kit (Geneaid Biotech. Ltd., Taipei, Taiwan). In addition, 42 tracheal samples from 42 local chicken (LC) flocks [3] (one trachea per flock) were collected in the field in Southern Taiwan and named TC1 to TC42.
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