Author: PIEWBANG, Chutchai; RUNGSIPIPAT, Anudep; POOVORAWAN, Yong; TECHANGAMSUWAN, Somporn
Title: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs Document date: 2016_9_15
ID: w3g6ohac_19
Snippet: Optimized and analytical performances of simplex and multiplex PCR assays: Optimization of each simplex PCR was undertaken using positive controls and clinical samples with different cycling conditions. Different annealing temperatures were evaluated, with the optimum Ta for all virus detections being 58°C, at which temperature no primer dimers or non-specific amplicons were detected (data not shown). In silico and in vitro analytical specificit.....
Document: Optimized and analytical performances of simplex and multiplex PCR assays: Optimization of each simplex PCR was undertaken using positive controls and clinical samples with different cycling conditions. Different annealing temperatures were evaluated, with the optimum Ta for all virus detections being 58°C, at which temperature no primer dimers or non-specific amplicons were detected (data not shown). In silico and in vitro analytical specificity tests revealed that each primer was able to amplify the specific target DNA without any cross amplification among the CIRDC viruses, CPV, CCoV and B. bronchiseptica. In addition, the sequenced amplicons showed 100% sequence identity with their respective corresponding sequence in the GenBank database.
Search related documents:
Co phrase search for related documents- PCR assay and primer dimer: 1, 2
- PCR assay and sequence amplicon: 1, 2, 3, 4
- PCR assay and sequence identity: 1, 2, 3, 4, 5, 6, 7, 8
- PCR assay and specificity test: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31
- PCR assay and target dna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- PCR assay and virus detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- PCR simplex optimization and simplex optimization: 1
- positive control and primer dimer: 1
- positive control and specific target dna: 1
- positive control and specificity test: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- positive control and target dna: 1, 2, 3
- positive control and virus detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- primer dimer and virus detection: 1
- sequence amplicon and target dna: 1
- sequence amplicon and virus detection: 1
- sequence identity and virus detection: 1, 2, 3, 4, 5
- specific target dna amplify and target dna: 1
- specific target dna and target dna: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28
- specific target dna and virus detection: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date