Selected article for: "database sequence and sequence identity"
Author: PIEWBANG, Chutchai; RUNGSIPIPAT, Anudep; POOVORAWAN, Yong; TECHANGAMSUWAN, Somporn
Title: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs
  • Document date: 2016_9_15
    • ID: w3g6ohac_19
    Snippet: Optimized and analytical performances of simplex and multiplex PCR assays: Optimization of each simplex PCR was undertaken using positive controls and clinical samples with different cycling conditions. Different annealing temperatures were evaluated, with the optimum Ta for all virus detections being 58°C, at which temperature no primer dimers or non-specific amplicons were detected (data not shown). In silico and in vitro analytical specificit.....
    Document: Optimized and analytical performances of simplex and multiplex PCR assays: Optimization of each simplex PCR was undertaken using positive controls and clinical samples with different cycling conditions. Different annealing temperatures were evaluated, with the optimum Ta for all virus detections being 58°C, at which temperature no primer dimers or non-specific amplicons were detected (data not shown). In silico and in vitro analytical specificity tests revealed that each primer was able to amplify the specific target DNA without any cross amplification among the CIRDC viruses, CPV, CCoV and B. bronchiseptica. In addition, the sequenced amplicons showed 100% sequence identity with their respective corresponding sequence in the GenBank database.

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