Selected article for: "analytical sensitivity and PCR detection"

Author: PIEWBANG, Chutchai; RUNGSIPIPAT, Anudep; POOVORAWAN, Yong; TECHANGAMSUWAN, Somporn
Title: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs
  • Document date: 2016_9_15
  • ID: w3g6ohac_20
    Snippet: Analytical sensitivity, specificity and reproducibility: The sensitivity of the multiplex PCR was tested by detection of the various viruses in serial dilutions and compared with that using the simplex PCR for each particular virus. The multiplex PCR products of the tested viruses were observed at the same template dilutions as with the simplex PCR, suggesting a similar sensitivity for the simplex and multiplex PCRs ( Figs. 1 and 2) . The highest.....
    Document: Analytical sensitivity, specificity and reproducibility: The sensitivity of the multiplex PCR was tested by detection of the various viruses in serial dilutions and compared with that using the simplex PCR for each particular virus. The multiplex PCR products of the tested viruses were observed at the same template dilutions as with the simplex PCR, suggesting a similar sensitivity for the simplex and multiplex PCRs ( Figs. 1 and 2) . The highest detection threshold was found for CDV and CRCoV, then CaHV-1 and CIV, and finally by CPIV and CAdV-2.

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