Selected article for: "real time and RT PCR real time PCR"
Author: Kim, Yeong Hoon; Lee, Jihoo; Kim, Young-Eun; Chong, Chom-Kyu; Pinchemel, Yanaihara; Reisdörfer, Francis; Coelho, Joyce Brito; Dias, Ronaldo Ferreira; Bae, Pan Kee; Gusmão, Zuinara Pereira Maia; Ahn, Hye-Jin; Nam, Ho-Woo
Title: Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus
  • Document date: 2018_2_28
    • ID: skzidybm_19
    Snippet: To investigate the analytical sensitivity of RDT, comparison with ELISA, plaque reduction neutralization test (PRNT) and PCR was performed on 30 Zika Positive Specimens provided by the Hospital Gaffree e Guinle (Rio de Janeiro, Brazil). Real-Time RT-PCR (RealStar Zika RT-PCR Kit and AccuPower ZIKA Real-Time RT-PCR Kit) is the only method officially approved and used as the gold standard diagnostic method. Supernatant samples (100 μl) or serum sa.....
    Document: To investigate the analytical sensitivity of RDT, comparison with ELISA, plaque reduction neutralization test (PRNT) and PCR was performed on 30 Zika Positive Specimens provided by the Hospital Gaffree e Guinle (Rio de Janeiro, Brazil). Real-Time RT-PCR (RealStar Zika RT-PCR Kit and AccuPower ZIKA Real-Time RT-PCR Kit) is the only method officially approved and used as the gold standard diagnostic method. Supernatant samples (100 μl) or serum samples (1 μl and sample diluent 99 μl) were applied to wells of the ELISA. A sample was considered positive if the optical density (OD) at 490 nm with a spectrophotometer (Perkin Elmer Victor3, Turku, Finland) was 2-fold greater than that of a blank (PBS) sample. The cut-off value was set at twice the average value of the blank sample in 3 replicates.

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