Title: Development of graft-vs.-host disease-like syndrome in cyclosporine- treated rats after syngeneic bone marrow transplantation. I. Development of cytotoxic T lymphocytes with apparent polyclonal anti-Ia specificity, including autoreactivity Document date: 1985_4_1
ID: rqggh4y2_5
Snippet: Marrow Transplantation. Donor animals were killed by CO2 asphyxiation. Marrow from femurs, tibias, and humeri was collected in Hank's solution supplemented with 50 U/ml penicillin and 50 ~g/ml streptomycin. The marrow cells were adjusted to a concentration of 6 × 107 nucleated cells/mi and 1 ml was infused into recipient animals by intravenous injection through the tail vein 1 d after irradiation......
Document: Marrow Transplantation. Donor animals were killed by CO2 asphyxiation. Marrow from femurs, tibias, and humeri was collected in Hank's solution supplemented with 50 U/ml penicillin and 50 ~g/ml streptomycin. The marrow cells were adjusted to a concentration of 6 × 107 nucleated cells/mi and 1 ml was infused into recipient animals by intravenous injection through the tail vein 1 d after irradiation.
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