Author: Azhar, Esam I.; Hashem, Anwar M.; El-Kafrawy, Sherif A.; Sohrab, Sayed Sartaj; Aburizaiza, Asad S.; Farraj, Suha A.; Hassan, Ahmed M.; Al-Saeed, Muneera S.; Jamjoom, Ghazi A.; Madani, Tariq A.
Title: Detection of the Middle East Respiratory Syndrome Coronavirus Genome in an Air Sample Originating from a Camel Barn Owned by an Infected Patient Document date: 2014_7_22
ID: ynlj4cge_9
Snippet: Sequencing and alignment. Further confirmation was performed by partially sequencing the UpE, ORF1a, ORF1b, RdRp, and N regions of the viral genome as per the WHO recommendations (23) . In addition, one region containing unique mutations in isolates obtained in our previous report (20) were also sequenced. Sequencing was performed as described previously (20) . Sequences were aligned with the genome of the MERS-CoV-Jeddah-camel-1isolate (KF917527.....
Document: Sequencing and alignment. Further confirmation was performed by partially sequencing the UpE, ORF1a, ORF1b, RdRp, and N regions of the viral genome as per the WHO recommendations (23) . In addition, one region containing unique mutations in isolates obtained in our previous report (20) were also sequenced. Sequencing was performed as described previously (20) . Sequences were aligned with the genome of the MERS-CoV-Jeddah-camel-1isolate (KF917527) obtained in our pervious study using Geneious 7.0.6 software.
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