Selected article for: "accession number and Genbank accession"
Author: TSAI, Cheng-Ta; TSAI, Hsin-Fu; WANG, Ching-Ho
Title: Detection of infectious bronchitis virus strains similar to Japan in Taiwan
  • Document date: 2016_1_29
    • ID: xvos1qto_4
    Snippet: The presence of IBV in the trachea was evaluated by RT-PCR using the N-specific primers NP1/NP2 [8] . The primer sequences for this gene are as follows: NP1: 5′-GGTA G (C/T) GG (C/T) GTTCCTGATA A-3′ (26029-26048, H120 numbering, GenBank accession number: FJ888351) and NP2: 5′-TCATCTTGTC (A/G) TCAC CAAAA-3′ (26647-26628). The RT-PCR was performed according to a previously described method [8] . The RT-PCR products produced with this primer.....
    Document: The presence of IBV in the trachea was evaluated by RT-PCR using the N-specific primers NP1/NP2 [8] . The primer sequences for this gene are as follows: NP1: 5′-GGTA G (C/T) GG (C/T) GTTCCTGATA A-3′ (26029-26048, H120 numbering, GenBank accession number: FJ888351) and NP2: 5′-TCATCTTGTC (A/G) TCAC CAAAA-3′ (26647-26628). The RT-PCR was performed according to a previously described method [8] . The RT-PCR products produced with this primer set were sequenced and compared. The RT-PCR products were sequenced at least twice from at least two RT-PCR products to make sure that the sequences were identical.

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