Author: PIEWBANG, Chutchai; RUNGSIPIPAT, Anudep; POOVORAWAN, Yong; TECHANGAMSUWAN, Somporn
Title: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs Document date: 2016_9_15
ID: w3g6ohac_10
Snippet: Optimizations of multiplex PCR: The multiplex PCR was optimized separately for RNA-and DNA-associated CIRDC viruses. The starting genetic material for RNA virus detection was derived from two compartments: (1) product from the first nested PCR of CRCoV and (2) cDNA of the other RNA viruses. Reaction composition and condition were optimized as mentioned above for the simplex PCR. The suitable Ta for all RNA and DNA viruses was selected for further.....
Document: Optimizations of multiplex PCR: The multiplex PCR was optimized separately for RNA-and DNA-associated CIRDC viruses. The starting genetic material for RNA virus detection was derived from two compartments: (1) product from the first nested PCR of CRCoV and (2) cDNA of the other RNA viruses. Reaction composition and condition were optimized as mentioned above for the simplex PCR. The suitable Ta for all RNA and DNA viruses was selected for further comparative analysis with simplex PCR.
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