Author: DOKI, Tomoyoshi; TAKANO, Tomomi; HOHDATSU, Tsutomu
Title: Development of a mouse-feline chimeric antibody against feline tumor necrosis factor-alpha Document date: 2016_6_4
ID: y0x0quha_7
Snippet: Cell cultures: FO mouse myeloma cells (ATCC CRL-1646) and WEHI-164 murine sarcoma cells (ATCC CRL1751) were obtained from the American Type Culture Collection. The hybridoma mAb 2-4 cell was developed in our laboratory [4] . FO cells and hybridoma mAb 2-4 cell producing the antibody to feline TNF-alpha were maintained in Dulbecco's modified Eagle's minimum essential medium supplemented with 10% FCS and antibiotics. WEHI-164 cells were maintained .....
Document: Cell cultures: FO mouse myeloma cells (ATCC CRL-1646) and WEHI-164 murine sarcoma cells (ATCC CRL1751) were obtained from the American Type Culture Collection. The hybridoma mAb 2-4 cell was developed in our laboratory [4] . FO cells and hybridoma mAb 2-4 cell producing the antibody to feline TNF-alpha were maintained in Dulbecco's modified Eagle's minimum essential medium supplemented with 10% FCS and antibiotics. WEHI-164 cells were maintained in RPMI 1640 growth medium supplemented with 10% FCS, antibiotics, 50 µM 2-mercaptoethanol and 2 µg/ml of polybrene.
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