Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus

Kim, Yeong Hoon; Lee, Jihoo; Kim, Young-Eun; Chong, Chom-Kyu; Pinchemel, Yanaihara; Reisdörfer, Francis; Coelho, Joyce Brito; Dias, Ronaldo Ferreira; Bae, Pan Kee; Gusmão, Zuinara Pereira Maia; Ahn, Hye-Jin; Nam, Ho-Woo

The cross-reaction with DENV represents 1 major disadvantage of the test. Presumed causes of cross-reaction of Zika RDT to DENV may be caused by mixed infection of ZIKV and DENV both in vectors and in human, and overlapping clinical symptoms make differential diagnosis a problem. ZIKV and DENV show a higher than 50% homology in E and NS1 proteins [38] , and these common antigenic determinants may confuse the issue. Pre-existing immunity to prior DENV infection might also increase ZIKV replication through antibodydependent enhancement (ADE) of ZIKV infection [40] causing variable results. In this context, cross-reactivity between DENV and ZIKV seems inevitable. Indeed, it has been suggested that ZIKV must be considered another member of the DENV serocomplex [40] .