Development of graft-vs.-host disease-like syndrome in cyclosporine- treated rats after syngeneic bone marrow transplantation. I. Development of cytotoxic T lymphocytes with apparent polyclonal anti-Ia specificity, including autoreactivity

In two other experiments, identical results were observed, with the minor exception that there was incomplete (~50%) recovery of cytotoxic T cell activity in the adherent fraction of cells panned with the W3/13 pan-T mAb. It seems likely that the cells were coated with the mAb, and that this interferred with lysis, suggesting close association with the antigen receptor. This hypothesis was further supported by the observation that addition of the pan-T mAb, but not the OX8 or W3/25 antibodies, to the CML assay resulted in significant inhibition of lysis. This appeared to be directed at the effector cell, since preincubation of targets with the pan-T mAb did not result in inhibition of lysis (see below).